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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Human Umbilical Artery Smooth Muscle Cells Huasmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Human Umbilical Artery Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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Effects of bare and coating-modified groups on SMCs proliferation. (A–C) Representative fluorescence images of HUASMCs cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Bioactive Materials

Article Title: A hierarchical MgF 2 /polyurethane/pitavastatin coating alleviates degradation and enhances endothelialization of bioresorbable magnesium alloy stents

doi: 10.1016/j.bioactmat.2025.08.038

Figure Lengend Snippet: Effects of bare and coating-modified groups on SMCs proliferation. (A–C) Representative fluorescence images of HUASMCs cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Human umbilical vein ECs (HUVECs), human umbilical artery SMCs (HUASMCs), and MΦs were obtained from Procell Life Science & Technology Co., Ltd. All cell lines were maintained and passaged in a cell culture incubator at 37 °C with 5 % CO 2 .

Techniques: Modification, Fluorescence, Control, Expressing, Comparison